Project

Bioprotection is an innovative alternative to the use of sulfites in winemaking. It relies on the use of non-Saccharomyces yeasts, particularly Metschnikowia pulcherrima, which are able to limit the growth of spoilage microorganisms. Although this strategy is now widely used, the molecular mechanisms underlying its effectiveness remain poorly understood. One of the interaction mechanisms potentially responsible for this phenomenon is the production of killer toxin(s) by M. pulcherrima. These protein-based compounds could be responsible for inhibiting the growth of spoilage microorganisms. The killer activity of a protein fraction has already been characterized within the laboratory. The aim of this internship will be to produce, purify, characterize, and identify the protein(s) associated with this key phenotypic trait.

Working Environment

The internship will be carried out at the interface of the Agroecology Joint Research Unit (IPM division) and the PAM Lab (Food and Microbiological Processes), in partnership with Lallemand Œnologie, a global leader in microbiological solutions for winemaking.

Responsibilities (6 months)

• Produce the killer protein(s) and optimize the conditions for their production;
• Implement various protein purification techniques (chromatography, ultrafiltration, precipitation, etc.);
• Assess the purity of protein fractions (SDS-PAGE, protein assays, spectrophotometry);
• Identify the protein(s) of interest;
• Evaluate their antimicrobial activity against different microorganisms;

• Analyze results and contribute to their scientific dissemination.

   

Candidate Profile

We are looking for a Master’s student in their second year (M2) or a final-year engineering student who is motivated, independent, and curious, with a background in biochemistry, biotechnology, microbiology, or food science. Knowledge of protein purification and characterization techniques, as well as microbiology, would be particularly appreciated and would be a valuable asset for this project.

Application 

Please send your CV and cover letter to:
Dr. Chloé ABRY – chloe.abry@u-bourgogne.fr
Pr. Hervé ALEXANDRE – rvalex@ube.fr